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Aoyagi K, Ohara-Imaizumi M, Itakura M, Torii S, Akimoto Y, Nishiwaki C, Nakamichi Y, Kishimoto T, Kawakami H, Harada A, Takahashi M, Nagamatsu S.

VAMP7 regulates autophagy to maintain mitochondrial homeostasis and to control insulin secretion in pancreatic beta-cells.

Diabetes, in press, 2016


Kim K, Oh CM, Ohara-Imaizumi M, Park S, Namkung J, Yadav VK, Tamarina NA, Roe MW, Philipson LH, Karsenty G, Nagamatsu S, German MS, Kim H.

Functional role of serotonin in insulin secretion in a diet-induced insulin-resistant state.

Endocrinology, 156: 444-52, 2015


Aoyagi K, Rossignol E, Hamdan FF, Mulcahy B, Xie L, Nagamatsu S, Rouleau GA, Zhen M, Michaud JL.

A gain-of-function mutation in NALCN in a child with intellectual disability, ataxia, and arthrogryposis.

Hum Mutat, 36: 753-7, 2015


Ohara-Imaizumi M, Kim H, Yoshida M, Fujiwara T, Aoyagi K, Toyofuku Y, Nakamichi Y, Nishiwaki C, Okamura T, Uchida T, Fujitani Y, Akagawa K, Kakei M, Watada H, German MS, Nagamatsu S.

Serotonin regulates glucose-stimulated insulin secretion from pancreatic beta cells during pregnancy.

Proc Natl Acad Sci USA, 110: 19420-5, 2013


Xie L, Gao S, Alcaire SM, Aoyagi K, Wang Y, Griffin JK, Stagljar I, Nagamatsu S, Zhen M.

NLF-1 delivers a sodium leak channel to regulate neuronal excitability and modulate rhythmic locomotion.

Neuron, 77: 1069-82, 2013


Aoyagi K, Ohara-Imaizumi M, Nishiwaki C, Nakamichi Y, Ueki K, Kadowaki T, Nagamatsu S.

Acute inhibition of PI3K-PDK1-Akt pathway potentiates insulin secretion through upregulation of newcomer granule fusions in pancreatic beta-cells.

PLoS One, 7: e47381, 2012


Nagamatsu S, Ohara-Imaizumi M, Nakamichi Y, Aoyagi K, Nishiwaki C.

DPP-4 inhibitor des-F-sitagliptin treatment increased insulin exocytosis from db/db mice beta cells.

Biochem Biophys Res Commun, 412: 556-60, 2011


Ohara-Imaizumi M, Yoshida M, Aoyagi K, Saito T, Okamura T, Takenaka H, Akimoto Y, Nakamichi Y, Takanashi-Yanobu R, Nishiwaki C, Kawakami H, Kato N, Hisanaga S, Kakei M, Nagamatsu S.

Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis.

PLoS One, 5: e15553, 2010


Aoyagi K, Ohara-Imaizumi M, Nishiwaki C, Nakamichi Y, Nagamatsu S.

Insulin/phosphoinositide 3-kinase pathway accelerates the glucose-induced first-phase insulin secretion through TrpV2 recruitment in pancreatic beta-cells.

Biochem J, 432: 375-86, 2010


Zhao A, Ohara-Imaizumi M, Brissova M, Benninger RK, Xu Y, Hao Y, Abramowitz J, Boulay G, Powers AC, Piston D, Jiang M, Nagamatsu S, Birnbaumer L, Gu G.

Gao represses insulin secretion by reducing vesicular docking in pancreatic beta-cells.

Diabetes, 59: 2522-9, 2010


Aoyagi K, Ohara-Imaizumi M, Nishiwaki C, Nakamichi Y, Nagamatsu S.

Glinide, but not sulfonylurea, can evoke insulin exocytosis by repetitive stimulation: imaging analysis of insulin exocytosis by secretagogue-induced repetitive stimulations.

Exp Diabetes Res, 20009: 278762, 2009


Ohara-Imaizumi M, Aoyagi K, Akimoto Y, Nakamichi Y, Nishiwaki C, Kawakami H, Nagamatsu S.

Imaging exocytosis of single glucagon-like peptide-1 containing granules in a murine enteroendocrine cell line with total internal reflection fluorescent microscopy.

Biochem Biophys Res Commun, 390: 16-20, 2009


Ohara-Imaizumi M, Aoyagi K, Nakamichi, Y, Nishiwaki C, Sakurai T, Nagamatsu S.

Pattern of rise in subplasma membrane Ca2+ concentration determines type of fusing insulin granules in pancreatic beta cells.

Biochem Biophys Res Commun, 385: 291-5, 2009


Akimoto Y, Sawada H, Ohara-Imaizumi M, Nagamatsu S, Kawakami H.

Change in long-spacing collagen in Descemets membrane of diabetic Goto-Kakizaki rats and its suppression by antidiabetic agents.

Exp Diabetes Res, 2008: 818341, 2008


Okamoto M, Ohara-Imaizumi M, Kubota N, Hashimoto S, Eto K, Kanno T, Kubota T, Wakui M, Nagai R, Noda M, Nagamatsu S, Kadowaki T.

Adiponectin induces insulin secretion in vitro and in vivo at a low glucose concentration.

Diabetologia, 51: 827-35, 2008


Kawai J, Ohara-Imaizumi M, Nakamichi Y, Okamura T, Akimoto Y, Matsushima S, Aoyagi K, Kawakami H, Watanabe T, Watada H, Kawamori R, Nagamatsu S.

Insulin exocytosis in GK rat beta cells subjected to long-term glinide or sulphonylurea treatment.

Biochem J, 412: 92-101, 2008


Ohara-Imaizumi M, Fujiwara T, Nakamichi Y, Okamura T, Akimoto Y, Kawai J, Matsushima S, Kawakami H, Watanabe T, Akagawa K, Nagamatsu S.

Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis. 

J Cell Biol, 177: 695-705, 2007


Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E.

EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.

Cell, 129(2): 359-70, 2007


Uchida T, Iwashita N, Ohara-Imaizumi M, Ogihara T, Nagai S, Choi JB, Tamura Y, Tada N, Kawamori R, Nakayama KI, Nagamatsu S, Watada H.

Protein kinase Cdelta plays a non-redundant role in insulin secretion in pancreatic beta cells. 

J Biol Chem, 282: 2707-16, 2007


 

Nagamatsu S, Ohara-Imaizumi M, Nakamichi Y, Kikuta T, Nishiwaki C.

Imaging docking and fusion of insulin granules induced by antidiabetes agents: sulfonylurea and glinide drugs preferentially mediate the fusion of newcomer, but not previously docked, insulin granules.

Diabetes, 55: 2819-25, 2006


Yamaguchi S, Katahira H, Ozawa S, Nakamichi Y, Tanaka T, Shimoyama T, Takahashi K, Yoshimoto K, Imaizumi MO, Nagamatsu S, Ishida H.
Activators of AMP-activated protein kinase enhance GLUT4 translocation and its glucose transport activity in 3T3-L1 adipocytes.

Am J Physiol Endocrinol Metab, 289: E643-9, 2005


M. Ohara-Imaizumi, T. Ohtsuka, S. Matsushima, Y. Akimoto, C. Nishiwaki, Y. Nakamichi, T. Kikuta, S. Nagai, H. Kawakami, T. Watanabe & S. Nagamatsu
ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic
β cells and functions in insulin exocytosis: Interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence microscopy.

Mol Biol Cell, 16: 3289-3300, 2005


T. Kikuta, M. Ohara-Imaizumi, M. Nakazaki, C. Nishiwaki, Y. Nakamichi, C. Tei, L. Aguilar-Bryan, J. Bryan & S. Nagamatsu
Docking and fusion of insulin secretory granules in SUR1 knock out mouse
β-cells observed by total internal reflection fluorescence microscopy.

FEBS Lett, 579: 1602-1606, 2005


K. Kasai, M. Ohara-Imaizumi, N. Takahashi, S. Mizutani, S. Zhao, T. Kikuta, H. Kasai, S. Nagamatsu, H. Gomi & T. Izumi
Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation.

J Clin Invest, 115: 388-396, 2005


M. Ohara-Imaizumi, C. Nishiwaki, Y. Nakamichi, T. Kikuta, S. Nagai & S. Nagamatsu
Corelation of syntaxin1 and SNAP-25 clusters with docking and fusion of insulin granules analyzed by total internal reflection microscopy.

Diabetologia 47: 2200-2207, 2004


M. Ohara-Imaizumi, A.K. Cardozo, T. Kikuta, D.L. Eizirik & S. Nagamatsu 
The cytokine IL-1
β reduces the docking and fusion of insulin granules in pancreatic β-cells, preferentially decreasting the first phase of exocytosis.

J Biol Chem, 279: 41271-41274, 2004


Y. Nakamichi, E. Wada, K. Aoki, M. Ohara-Imaizumi, T. Kikuta, C. Nishiwaki, S. Matsushima, T. Watanabe, K. Waka & S. Nagamatsu
Functions or pancreatic beta cells and adipocytes in bombesin receptor subtype-3-deficient mice.

Biochem Biophys Res Commun, 318: 698-703, 2004


M. Ohara-Imaizumi, C. Nishiwaki, T. Kikuta, S. Nagai, Y. Nakamichi & S. Nagamatsu
TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic
β-cells: different behaviour of granule motion between normal and Goto-Kakizaki diabetic rat β-cells.

Biochem J, 381; 13-18, 2004


S. Torii, T. Takeuchi, S. Nagamatsu & T. Izumi
Rab27 Effector Granuphilin Promotes the Plasma Membrane Targeting of Insulin Granules via Interaction with Syntaxin 1a.

J Biol Chem, 279; 22532-22538, 2004


M. Ohara-Imaizumi, C. Nishiwaki, T. Kikuta, K. Kumakura, Y. Nakamichi & S. Nagamatsu
Site of Docking and Fusion of Insulin Secretory Granules in Live MIN6 Cells Analyzed by TAT-conjugated Anti-syntaxin 1 Antibody and Total Internal Reflection Fluorescence Microscopy .

J Biol Chem, 279: 8403-8408, 2004


Y. Nakamichi, T. Kikuta, E. Ito, M. Ohara-Imaizumi, C. Nishiwaki, H. Ishida & S. Nagamatsu
PPAR-gamma overexpression suppresses glucose-induced proinsulin biosynthesis and insulin release synergistically with pioglitazone in MIN6 cells.

Biochem Biophys Res Commun, 306; 832-836, 2003.


J.W. Taraska, D. Perrais, M. Ohara-Imaizumi, S. Nagamatsu & W. Almers
Secretory granules are recaptured largely intact after stimulated exocytosis in cultured endocrine cells.

Proc Natl Acad Sci USA, 100: 2070-2075, 2003


Y. Nakamichi, M. Ohara-Imaizumi, H. Ishida & S. Nagamatsu
An Insulin-related peptide expressed in 3T3L1 adipocytes is localized in GLUT4 vesicles and secreted in response to exogenous insulin, which augments the insulin-stimulated glucose uptake.

J Cell Sci, 116: 73-79, 2003


M. Ohara-Imaizumi, Y. Nakamichi, C. Nishiwaki & S. Nagamatsu
Transduction of MIN6 beta Cells with TAT-Syntaxin SNARE Motif Inhibits Insulin Exocytosis in Biphasic Insulin Release in a Distinct Mechanism Analyzed by Evanescent Wave Microscopy.

J Biol Chem, 277: 50805-50811, 2002


M. Ohara-Imaizumi, Y. Nakamichi, T. Tanaka, H. Katsuta, H. Ishida & S. Nagamatsu
Monitoring of exocytosis and endocytosis of insulin secretory granules in the pancreatic
β-cell line MIN6 using pH-sensitive green fluorescent protein (pH luorin) and confocal laser microscopy.

Biochem J, 363: 73-80, 2002


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最近の代表的な総説・著書


Aoyagi K, Ohara-Imaizumi M, Nagamatsu S.

Regulation of resident and newcomer insulin granules by calcium and SNARE proteins.

Front Biosci, 16: 1197-210, 2011


Nagamatsu S, Ohara-Imaizumi M.

Imaging exocytosis of single insulin secretory granules with TIRF microscopy.

Methods Mol Biol, 440: 259-68, 2008


Nagamatsu S, Ohara-Imaizumi M.

IP7 debut in insulin release. 

Science, 318: 1249-50, 2007


永松信哉
膵β細胞におけるインスリン分泌機構について 
実験治療 674251-674256, 2004


永松信哉
β細胞のインスリン分泌開口放出機構 
糖尿病カレントライブラリー インスリン分泌(編者:岡芳知)東京 文光堂 p33-38, 2004


今泉美佳、永松信哉
インスリン開口放出の可視化(ミニレビュー)
生化学 7549-54,2003


永松信哉
ホルモン分泌の生化学的機構、
内分泌・糖尿病科 17:519-525, 2003


S. Nagamatsu and M. Ohara-Imaizumi
SNARE-mediated insulin exocytosis. In:
Recent research development in biophysics & biochemistry, Pandalai SG(ed.),
Research Signpost Publisher vol.3: 457-470, 2003.


永松信哉、今泉美佳
インスリン開口放出機構とSNARE蛋白質
日本臨床 60: 213-218, 2002.


永松信哉
インスリン開口放出の可視化と2−コンパートメントモデル
糖尿病学2002(編者:岡芳知)東学、診断と治療社、p92-104, 2002


今泉美佳、菊田敏輝、西脇知世乃、中道洋子
PowerPoint
で大判ポスターをつくってみよう!
実験医学 22: 2676-2681, 2004


今泉美佳
ポスター発表はチャンスの宝庫 ! 一歩進んだ発表のための計画・準備から当日のプレゼンまで  
羊土社 2003, 6


 

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