{"id":486,"date":"2021-04-01T13:00:24","date_gmt":"2021-04-01T04:00:24","guid":{"rendered":"http:\/\/www.kyorin-u.ac.jp\/univ\/user\/medicine\/fcm\/wordpress\/?page_id=486"},"modified":"2021-04-01T13:31:53","modified_gmt":"2021-04-01T04:31:53","slug":"facscalibur","status":"publish","type":"page","link":"https:\/\/www.kyorin-u.ac.jp\/univ\/user\/medicine\/fcm\/facscalibur","title":{"rendered":"BD FACSCalibur"},"content":{"rendered":"
\n
\n

FACSCalibur 4A \uff08analyzer\uff09<\/span><\/h2>\n<\/div>\n

\u203b\u672c\u6a5f\u306f\u904b\u7528\u3092\u7d42\u4e86\u3057\u307e\u3057\u305f\u3002\u5f8c\u7d99\u6a5f\u7a2e\u306eBD FACSLyric<\/a>\u3092\u3054\u5229\u7528\u4e0b\u3055\u3044\u3002<\/strong><\/span><\/p>\n

BD FACSCalibur\u306f\u3001\u9ad8\u6027\u80fd4\u30ab\u30e9\u30fc\u30d9\u30f3\u30c1\u30c8\u30c3\u30d7\u578b\u89e3\u6790\u5c02\u7528\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002\u30b3\u30f3\u30d1\u30af\u30c8\u3067\u64cd\u4f5c\u304c\u7c21\u5358\u306aBD FACSCalibur\u306b\u3088\u308a\u3001\u7d30\u80de\u8868\u9762\u6297\u539f\u89e3\u6790\u3001\u7d30\u80de\u5468\u671f\u89e3\u6790\u3001\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u89e3\u6790\u306a\u3069\u306e\u5f93\u6765\u304b\u3089\u306e\u7814\u7a76\u624b\u6cd5\u3092\u8fc5\u901f\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u52a0\u3048\u3066\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3001\u30de\u30eb\u30c1\u30ab\u30e9\u30fc\u89e3\u6790\u3001\u7d30\u80de\u5185\u6297\u539f\u3092\u306f\u3058\u3081\u3068\u3059\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u306a\u3069\u3001\u7cbe\u5de7\u306a\u6280\u8853\u3092\u8981\u3059\u308b\u7d30\u80de\u5de5\u5b66\u5206\u91ce\u306e\u7814\u7a76\u306b\u3082\u5e83\u304f\u7528\u3044\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u96fb\u6e90\u6295\u5165\u304b\u3089\u308f\u305a\u304b10\u5206\u3067\u89e3\u6790\u304c\u53ef\u80fd\u306a\u305f\u3081\u3001FCM\u306e\u5165\u9580\u6a5f\u30fb\u89e3\u6790\u5c02\u7528\u6a5f\u3068\u3057\u3066\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u57fa\u672cSPEC<\/h3>\n\n\n\n\n\n\n
\n
Function<\/div>\n<\/td>\n
\n
\u5185\u3000\u3000\u5bb9<\/div>\n<\/td>\n<\/tr>\n
\n
\u30bd\u30fc\u30c6\u30a3\u30f3\u30b0<\/div>\n<\/td>\n
\n
\u4e0d\u53ef<\/div>\n<\/td>\n<\/tr>\n
\n
\u5149\u8ef8\u8abf\u6574<\/div>\n<\/td>\n
\n
\u4e0d\u8981<\/div>\n<\/td>\n<\/tr>\n
\n
\u96e3\u6613\u5ea6<\/div>\n<\/td>\n
\n
\u5bb9\u6613<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u30ec\u30fc\u30b6\u30fc<\/h3>\n\n\n\n\n\n
\n
Excitation wave<\/div>\n<\/td>\n
\n
\u642d\u8f09\u30ec\u30fc\u30b6\u30fc<\/div>\n<\/td>\n<\/tr>\n
\n
488nm<\/div>\n<\/td>\n
\n
\u7a7a\u51b7\u30a2\u30eb\u30b4\u30f3\u30ec\u30fc\u30b6\u30fc<\/div>\n<\/td>\n<\/tr>\n
\n
635nm<\/div>\n<\/td>\n
\n
\u534a\u5c0e\u4f53\u8d64\u8272\u30ec\u30fc\u30b6\u30fc<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u691c\u51fa\u3067\u304d\u308b\u86cd\u5149\u8272\u7d20<\/h3>\n<\/div>\n

\n\n\n\n\t\n\n\t\n\t\n\t\n\t
Excitation wave<\/th>Emission wave range<\/th>Fluorescent dye\t<\/th>\n<\/tr>\n<\/thead>\n
488nm (B)<\/td>Green 520\/50<\/td>FL1: FITC, Alexa Fluor 488, eFluor 520, Horizon BB515<\/td>\n<\/tr>\n
<\/td>Yellow 582\/15<\/td>FL2: PE, Alexa Fluor 546, PI<\/td>\n<\/tr>\n
<\/td>Red 710\/50<\/td>FL3: PE-Cy5, PerCP, 7AAD<\/td>\n<\/tr>\n
633nm (R)<\/td>Red 661\/16<\/td>FL4: APC, Alexa Fluor 647<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/p>\n
\n

\u86cd\u5149\u8272\u7d20\u306f\u4e00\u3064\u306e\u691c\u51fa\u5668\u306b\u3064\u304d\u3001\u4e00\u7a2e\u985e\u3060\u3051\u691c\u51fa\u53ef\u80fd\u3067\u3059\u3002\u4f8b\u3048\u3070\u3001FITC\u3068Alexa
\n488\u3092\u540c\u6642\u306b\u67d3\u8272\u3057\u3066\u3082\u898b\u5206\u3051\u308b\u4e8b\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3059\u306a\u308f\u3061FACSCalibur\u3067\u306f4\u8272\u307e\u3067\u540c\u6642\u306b\u89e3\u6790\u304c\u53ef\u80fd\u3067\u3059\u3002\u306a\u304a\u3001\u8272\u7d20\u306b\u3088\u3063\u3066\u306f\u86cd\u5149\u767a\u5149\u304c\u4ed6\u306e\u691c\u51fa\u5668\u3067\u3082\u691c\u51fa\u3055\u308c\u308b\u5834\u5408\u304c\u3042\u308b\u305f\u3081\u3001\u6e2c\u5b9a\u6642\u306b\u8abf\u6574\u304c\u5fc5\u8981\u3067\u3059\uff08\u30b3\u30f3\u30da\u30f3\u30bc\u30fc\u30b8\u30e7\u30f3\uff09\u3002<\/p>\n

\u6ce8\u610f1\uff1aBD Fluorescence Spectrum Viewer<\/a>\u3092\u4f7f\u3046\u3068\u3001\u5f53\u8a72\u6a5f\u5668\u3067\u4f7f\u7528\u53ef\u80fd\u306a\u8272\u7d20\u3092\u8abf\u3079
\n\u308b\u4e8b\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u6ce8\u610f2\uff1a\u86cd\u5149\u8272\u7d20\u306b\u306f\u3044\u304f\u3064\u304b\u306e\u7a2e\u985e\u306e\u7d30\u80de\u3078\u7d50\u5408\u3057\u3084\u3059\u3044\u3082\u306e\u304c\u3042\u308a\u3001Cy3\u3001Cy5\u3001
\nCy5.5\u3001Cy7\u304a\u3088\u3073TexasRed\u3067\u76f4\u63a5\u7684\u306b\u6297\u4f53\u306b\u6a19\u8b58\u3055\u308c\u305f\u3082\u306e\u3001\u30bf\u30f3\u30c7\u30e0\u8272\u7d20\u6a19\u8b58\u306e\u4e00\u90e8\u306f\u305d\u306e\u50be\u5411\u304c\u3042\u308b\u3002Cy5\u306fFc\u30ec\u30bb\u30d7\u30bf\u30fc\u306b\u4f4e\u3044\u89aa\u548c\u6027\u3092\u3082\u3061\u3001PE-Cy5\u30bf\u30f3\u30c7\u30e0\u8272\u7d20\u3082\u540c\u69d8\u306a\u7279\u6027\u304c\u3042\u308b\u306e\u3067Monocyte\u7cfb\u306e\u7d30\u80de\u306e\u67d3\u8272\u6642\u306f\u6ce8\u610f\u3002Cy5.5\u3082Fc\u30ec\u30bb\u30d7\u30bf\u30fc\u306b\u4f4e\u3044\u89aa\u548c\u6027\u3092\u3082\u3064\u304c\u3001PerCP-Cy5.5\u30bf\u30f3\u30c7\u30e0\u8272\u7d20\u306f\u540c\u69d8\u306a\u7279\u6027\u3092\u3082\u3063\u3066\u3044\u306a\u3044\u3002<\/p>\n

\u6ce8\u610f3\uff1aPE-Cy5\u3001PE-Cy7\u3001PE-TR\u3001PerCP-Cy5.5\u3001APC-Cy7\u306a\u3069\u30bf\u30f3\u30c7\u30e0\u8272\u7d20\u3092\u4f7f\u7528\u3059\u308b\u6642\u306f\u6ce8\u610f\u304c\u5fc5\u8981\u3002\u30bf\u30f3\u30c7\u30e0\u8272\u7d20\u3068\u306f2\u3064\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u304c\u30ab\u30c3\u30d7\u30ea\u30f3\u30b0\u3057\u305f\u8272\u7d20\u3067\u30011\u3064\u304c\u30ec\u30fc\u30b6\u30fc\u5149\u3092\u5438\u53ce\u3057\u3001\u3082\u3046\u7247\u65b9\u306e\u8272\u7d20\u306b\u305d\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u4f1d\u9054\u3059\u308b\u3002\u4f8b\u3048\u3070\u3001PE-Cy5\u306e\u5834\u5408488 nm\u306e\u30ec\u30fc\u30b6\u30fc\u306e\u5438\u53ce\u3092PE\u304c\u884c\u306a\u3044\u3001Cy5\u304c\u305d\u306e\u30a8\u30cd\u30eb\u30ae\u30fc\u3092\u53d7\u3051\u53d6\u308a\u3001Cy5\u306e\u86cd\u5149\u7279\u6027\u306b\u3088\u308a\u86cd\u5149\u3092\u767a\u3059\u308b\u3002\u901a\u5e38\u306f\u30ec\u30fc\u30b6\u30fc\u5149\u3092\u53d7\u3051\u53d6\u308b\u86cd\u5149\u8272\u7d20\u305d\u306e\u3082\u306e\u3088\u308a\u660e\u308b\u304f\u306a\u308b\u304c\u3001\u6f5c\u5728\u7684\u306b\u86cd\u5149\u6ce2\u9577\u304c\u308f\u305a\u304b\u306b\u5909\u52d5\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u3001\u307e\u305f\u30ed\u30c3\u30c8\u3054\u3068\u306b\u86cd\u5149\u30aa\u30fc\u30d0\u30fc\u30e9\u30c3\u30d7\u304c\u7570\u306a\u308b\u30022\u672c\u4ee5\u4e0a\u306e\u30ec\u30fc\u30b6\u30fc\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u30012\u672c\u76ee\u4ee5\u964d\u306e\u30ec\u30fc\u30b6\u30fc\u3067\u52b1\u8d77\u3055\u308c\u306a\u3044\u304b\u3069\u3046\u304b\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u5fc5\u8981\u3002<\/p>\n<\/div>\n

\u30ef\u30fc\u30af\u30b9\u30c6\u30fc\u30b7\u30e7\u30f3SPEC<\/h2>\n

\"pmg5.jpg\"<\/p>\n

Apple MacPro<\/p>\n

OS MacOS X<\/p>\n

Software<\/h3>\n

\"cellquestpro.jpg\" CellQuestPro<\/p>\n

\"facscomp.jpg\" FACSComp<\/p>\n

\"Automator.jpg\"ModFit LT for Mac V3.0<\/p>\n

\"Synchronize.jpg\" Synchronize! Pro X<\/p>\n

\"Automator.jpg\" Automator<\/p>\n

\u4f7f\u7528\u53ef\u80fdMedia<\/h3>\n

USB Memory
\nCD-R
\nCD-RW
\nDVD-R
\nNetwork<\/p>\n","protected":false},"excerpt":{"rendered":"

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