研究業績
2024
- Aoyagi K, Nishiwaki C, Nakamichi Y, Yamashita SI, Kanki T, Ohara-Imaizumi M
Imeglimin mitigates the accumulation of dysfunctional mitochondria to restore insulin secretion and suppress apoptosis of pancreatic β-cells from db/db mice.
Sci Rep, 14: 6178 [PubMed]
- 今泉美佳
『医学部基礎医学系教室の最前線 - 第５回 細胞生化学教室』
研究紹介：イメージングによるインスリン分泌機構の解明
杏林医学会雑誌, 55: 7-13
- 青柳共太、今泉美佳
２相性インスリン開口分泌のメカニズム
Diabetes Journal, 51: 44-52
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2023
- Aoyagi K, Yamashita SI, Akimoto Y, Nishiwaki C, Nakamichi Y, Udagawa H, Abe M, Sakimura K, Kanki T, Ohara-Imaizumi M
A new beta cell specific-mitophagy reporter mouse shows that metabolic stress leads to accumulation of dysfunctional mitochondria despite increased mitophagy.
Diabetologia, 66: 147-162 [PubMed]
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2020
- Udagawa H, Hiramoto M, Kawaguchi M, Uebanso T, Ohara-Imaizumi M, Nammo T, Nishimura W, Yasuda K.
Characterization of the taste receptor-related G-protein α-gustducin in pancreatic β-cells.
J Diabetes Invest, 11: 814-22 [PubMed]
- 青柳共太, 今泉美佳
２相性インスリン分泌のメカニズム
月刊糖尿病, 127: 20-26
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2019
- Ohara-Imaizumi M, Aoyagi K, Ohtsuka T
Role of the active zone protein, ELKS, in insulin secretion from pancreatic β-cells.
Mol Metab, 27: S81-S91 [PubMed]
- Ohara-Imaizumi M, Aoyagi K, Yamauchi H, Yoshida M, Mori MX, Hida Y, Ha TN, Ohkura M, Abe M, Akimoto Y, Nakamichi Y, Nishiwaki C, Kawakami H, Hara K, Sakimura K, Nagamatsu S, Mori Y, Nakai J, Kakei M, Ohtsuka T
ELKS/Voltage-dependent Ca2+ channel-β subunit module regulates polarized Ca2+ influx in pancreatic β-cells
Cell Reports, 26(5): 1213-26 [PubMed]
- Araki K, Araki A, Honda D, Izumoto T, Hashizume A, Hijikata Y, Yamada S, Iguchi Y, Hara A, Ikumi K, Kawai K, Ishigaki S, Nakamichi Y, Tsunekawa S, Seino Y, Yamamoto A, Takayama Y, Hidaka S, Tominaga M, Ohara-Imaizumi M, Suzuki A, Ishiguro H, Enomoto A, Yoshida M, Arima H, Muramatsu S, Sobue G, Katsuno M
TDP-43 regulates early-phase insulin secretion via CaV1.2-mediated exocytosis in islets
J Clin Invest, 136: 1371-7 [PubMed]
- 今泉美佳
膵β細胞のアクティブゾーンタンパク質ELKSが血管方向へのインスリン極性分泌をコントロールする
Diabetes J, 47: 168-70
- 今泉美佳
アクティブゾーンタンパク質ELKSによるインスリン極性分泌調節機構
Diabetes Strategy, 9: 156-7
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2018
- Aoyagi K, Itakura M, Fukutomi T, Nishiwaki C, Nakamichi Y, Torii S, Makiyama T, Harada A, Ohara-Imaizumi M
VAMP7 regulates autophagosome formation by supporting Atg9a functions in pancreatic β-cells from male mice.
Endocrinol, 159(11): 3674-88 [PubMed]
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2017
- Krishnankutty A, Kimura T, Saito T, Aoyagi K, Asada A, Takahashi SI, Ando K, Ohara-Imaizumi M, Ishiguro K, Hisanaga S.
In vitro regulation of glycogen synthase kinase 3β activity in neurons and braines.
Sci Rep, 7(1): 8602 [PubMed]
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2016
- Aoyagi K, Ohara-Imaizumi M, Itakura M, Torii S, Akimoto Y, Nishiwaki C, Nakamichi Y, Kishimoto T, Kawakami H, Harada A, Takahashi M, Nagamatsu S.
VAMP7 regulates autophagy to maintain mitochondrial homeostasis and to control insulin secretion in pancreatic β-cells.
Diabetes 65(6): 1648-59 [PubMed]
- Kunii M, Ohara-Imaizumi M, Takahashi N, Kobayashi M, Kawakami R, Kondoh Y, Shimizu T, Simizu S, Lin B, Nunomura K, Aoyagi K, Ohno M, Ohmuraya M, Sato T, Yoshimura SI, Sato K, Harada R, Kim YJ, Osada H, Nemoto T, Kasai H, Kitamura T, Nagamatsu S, Harada A.
Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells.
J Cell Biol 215(1): 121-138 [PubMed]
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2015
- Aoyagi K, Rossignol E, Hamdan FF, Mulcahy B, Xie L, Nagamatsu S, Rouleau GA, Zhen M, Michaud JL.
A gain-of-function mutation in NALCN in a child with intellectual disability, ataxia, and arthrogryposis.
Hum Mutat 36(8): 753-7 [PubMed]
- Kim K, Oh CM, Ohara-Imaizumi M, Park S, Namkung J, Yadav VK, Tamarina NA, Roe MW, Philipson LH, Karsenty G, Nagamatsu S, German MS, Kim H.
Functional role of serotonin in insulin secretion in a diet-induced insulin-resistant state.
Endocrinology 156(2): 444-52 [PubMed]
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2013
- Ohara-Imaizumi M, Kim H, Yoshida M, Fujiwara T, Aoyagi K, Toyofuku Y, Nakamichi Y, Nishiwaki C, Okamura T, Uchida T, Fujitani Y, Akagawa K, Kakei M, Watada H, German MS, Nagamatsu S.
Serotonin regulates glucose-stimulated insulin secretion from pancreatic β cells during pregnancy.
Proc Natl Acad Sci USA 110(48): 19420-5 [PubMed]
- Xie L, Gao S, Alcaire SM, Aoyagi K, Wang Y, Griffin JK, Stagljar I, Nagamatsu S, Zhen M.
NLF-1 delivers a sodium leak channel to regulate neuronal excitability and modulate rhythmic locomotion.
Neuron 77(6): 1069-82 [PubMed]
- Ando K, Kudo Y, Aoyagi K, Ishikawa R, Igarashi M, Takahashi M.
Calmodulin-dependent regulation of neurotransmitter release differs in subsets of neuronal cells.
Brain Res 1535: 1-13 [PubMed]
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2012
- Aoyagi K, Ohara-Imaizumi M, Nishiwaki C, Nakamichi Y, Ueki K, Kadowaki T, Nagamatsu S.
Acute inhibition of PI3K-PDK1-Akt pathway potentiates insulin secretion through upregulation of newcomer granule fusions in pancreatic β-cells.
PLoS One 7(10): e47381 [PubMed]
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2011
- Nagamatsu S, Ohara-Imaizumi M, Nakamichi Y, Aoyagi K, Nishiwaki C.
DPP-4 inhibitor des-F-sitagliptin treatment increased insulin exocytosis from db/db mice β cells.
Biochem Biophys Res Commun 412(4): 556-50 [PubMed]
- Aoyagi K, Ohara-Imaizumi M, Nagamatsu S.
Regulation of resident and newcomer insulin granules by calcium and SNARE proteins.
Front Biosci (Landmark Ed) 16: 1197-210 [PubMed]
- Sasakawa N, Ohara-Imaizumi M, Fukuda M, Kabayama H, Mikoshiba K, Kumakura K.
Dissociation of inositol polyphosphates from the C2B domain of synaptotagmin facilitates spontaneous release of catecholamines in adrenal chromaffin cells. A suggestive evidence of a fusion clamp by synaptotagmin.
Neuropharmacology 60(7-8): 1364-70 [PubMed]
- Yasuda K, Itakura M, Aoyagi K, Sugaya T, Nagata E, Ihara H, Takahashi M.
PKC-dependent inhibition of Ca2+-dependent exocytosis from astrocytes.
Glia 59(1): 143-51 [PubMed]
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2010
- Ohara-Imaizumi M, Yoshida M, Aoyagi K, Saito T, Okamura T, Takenaka H, Akimoto Y, Nakamichi Y, Takanashi-Yanobu R, Nishiwaki C, Kawakami H, Kato N, Hisanaga S, Kakei M, Nagamatsu S.
Deletion of CDKAL1 affects mitochondrial ATP generation and first-phase insulin exocytosis.
PLoS One 5(12): e15553 [PubMed]
- Aoyagi K, Ohara-Imaizumi M, Nishiwaki C, Nakamichi Y, Nagamatsu S.
Insulin/phosphoinositide 3-kinase pathway accelerates the glucose-induced first-phase insulin secretion through TrpV2 recruitment in pancreatic β-cells.
Biochem J 432(2): 375-86 [PubMed]
- Zhao A, Ohara-Imaizumi M, Brissova M, Benninger RK, Xu Y, Hao Y, Abramowitz J, Boulay G, Powers AC, Piston D, Jiang M, Nagamatsu S, Birnbaumer L, Gu G.
Gαo represses insulin secretion by reducing vesicular docking in pancreatic beta-cells.
Diabetes 59(10): 2522-9 [PubMed]
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2009
- Ohara-Imaizumi M, Aoyagi K, Akimoto Y, Nakamichi Y, Nishiwaki C, Kawakami H, Nagamatsu S.
Imaging exocytosis of single glucagon-like peptide-1 containing granules in a murine enteroendocrine cell line with total internal reflection fluorescent microscopy.
Biochem Biophys Res Commun 390(1): 16-20 [PubMed]
- Ohara-Imaizumi M, Aoyagi K, Nakamichi Y, Nishiwaki C, Sakurai T, Nagamatsu S.
Pattern of rise in subplasma membrane Ca2+ concentration determines type of fusing insulin granules in pancreatic beta cells.
Biochem Biophys Res Commun 385(3): 291-5 [PubMed]
- Aoyagi K, Ohara-Imaizumi M, Nishiwaki C, Nakamichi Y, Nagamatsu S.
Glinide, but not sulfonylurea, can evoke insulin exocytosis by repetitive stimulation: imaging analysis of insulin exocytosis by secretagogue-induced repetitive stimulations.
Exp Diabetes Res 2009: 278762 [PubMed]
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2008
- Kawai J, Ohara-Imaizumi M, Nakamichi Y, Okamura T, Akimoto Y, Matsushima S, Aoyagi K, Kawakami H, Watanabe T, Watada H, Kawamori R, Nagamatsu S.
Insulin exocytosis in Goto-Kakizaki rat beta-cells subjected to long-term glinide or sulfonylurea treatment.
Biochem J 412(1): 93-101 [PubMed]
- Akimoto Y, Sawada H, Ohara-Imaizumi M, Nagamatsu S, Kawakami H.
Change in long-spacing collagen in Descemet's membrane of diabetic Goto-Kakizaki rats and its suppression by antidiabetic agents.
Exp Diabetes Res 2008: 818341 [PubMed]
- Nagamatsu S, Ohara-Imaizumi M.
Imaging exocytosis of single insulin secretory granules with TIRF microscopy.
Methods Mol Biol 440: 259-68 [PubMed]
- Okamoto M, Ohara-Imaizumi M, Kubota N, Hashimoto S, Eto K, Kanno T, Kubota T, Wakui M, Nagai R, Noda M, Nagamatsu S, Kadowaki T.
Adiponectin induces insulin secretion in vitro and in vivo at a low glucose concentration.
Diabetologia 51(5): 827-35 [PubMed]
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2007
- Ohara-Imaizumi M, Fujiwara T, Nakamichi Y, Okamura T, Akimoto Y, Kawai J, Matsushima S, Kawakami H, Watanabe T, Akagawa K, Nagamatsu S.
Imaging analysis reveals mechanistic differences between first- and second-phase insulin exocytosis.
J Cell Biol 177(4): 695-705 [PubMed]
- Nagamatsu S, Ohara-Imaizumi M.
Cell biology. IP7 debut in insulin release.
Science 318(5854): 1249-50 [PubMed]
- Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E.
EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets.
Cell 129(2): 359-70 [PubMed]
- Uchida T, Iwashita N, Ohara-Imaizumi M, Ogihara T, Nagai S, Choi JB, Tamura Y, Tada N, Kawamori R, Nakayama KI, Nagamatsu S, Watada H.
Protein kinase Cdelta plays a non-redundant role in insulin secretion in pancreatic beta cells.
J Biol Chem 282(4): 2707-16 [PubMed]
- Akimoto Y, Hart GW, Wells L, Vosseller K, Yamamoto K, Munetomo E, Ohara-Imaizumi M, Nishiwaki C, Nagamatsu S, Hirano H, Kawakami H.
Elevation of the post-translational modification of proteins by O-linked N-acetylglucosamine leads to deterioration of the glucose-stimulated insulin secretion in the pancreas of diabetic Goto-Kakizaki rats.
Glycobiology 17(2): 127-40 [PubMed]
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2006
- Nagamatsu S, Ohara-Imaizumi M, Nakamichi Y, Kikuta T, Nishiwaki C.
Imaging docking and fusion of insulin granules induced by antidiabetes agents: sulfonylurea and glinide drugs preferentially mediate the fusion of newcomer, but not previously docked, insulin granules.
Diabetes 55(10): 2819-25 [PubMed]
- Ohara-Imaizumi M, Nagamatsu S.
Insulin exocytotic mechanism by imaging technique.
J Biochem 140(1): 1-5 [PubMed]
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2005
- Kikuta T, Ohara-Imaizumi M, Nakazaki M, Nishiwaki C, Nakamichi Y, Tei C, Aguilar-Bryan L, Bryan J, Nagamatsu S.
Docking and fusion of insulin secretory granules in SUR1 knock out mouse beta-cells observed by total internal reflection fluorescence microscopy.
FEBS Lett 579(7): 1602-6 [PubMed]
- Kasai K, Ohara-Imaizumi M, Takahashi N, Mizutani S, Zhao S, Kikuta T, Kasai H, Nagamatsu S, Gomi H, Izumi T.
Rab27a mediates the tight docking of insulin granules onto the plasma membrane during glucose stimulation.
J Clin Invest 115(2): 388-96 [PubMed]
- Yamaguchi S, Katahira H, Ozawa S, Nakamichi Y, Tanaka T, Shimoyama T, Takahashi K, Yoshimoto K, Imaizumi MO, Nagamatsu S, Ishida H.
Activators of AMP-activated protein kinase enhance GLUT4 translocation and its glucose transport activity in 3T3-L1 adipocytes.
Am J Physiol Endocrinol Metab 289(4): E643-9 [PubMed]
- Akishita M, Nagai K, Xi H, Yu W, Sudoh N, Watanabe T, Ohara-Imaizumi M, Nagamatsu S, Kozaki K, Horiuchi M, Toba K.
Renin-angiotensin system modulates oxidative stress-induced endothelial cell apoptosis in rats.
Hypertension 45(6): 1188-93 [PubMed]
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2004
- Ohara-Imaizumi M, Nishiwaki C, Kikuta T, Kumakura K, Nakamichi Y, Nagamatsu S.
Site of docking and fusion of insulin secretory granules in live MIN6 beta cells analyzed by TAT-conjugated anti-syntaxin 1 antibody and total internal reflection fluorescence microscopy.
J Biol Chem 279(9): 8403-8 [PubMed]
- Ohara-Imaizumi M, Nishiwaki C, Kikuta T, Nagai S, Nakamichi Y, Nagamatsu S.
TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic beta-cells: different behaviour of granule motion between normal and Goto-Kakizaki diabetic rat beta-cells.
Biochem J 381(Pt1): 13-8 [PubMed]
- Ohara-Imaizumi M, Cardozo AK, Kikuta T, Eizirik DL, Nagamatsu S.
The cytokine interleukin-1beta reduces the docking and fusion of insulin granules in pancreatic beta-cells, preferentially decreasing the first phase of exocytosis.
J Biol Chem 279(40): 41271-4 [PubMed]
- Nakamichi Y, Wada E, Aoki K, Ohara-Imaizumi M, Kikuta T, Nishiwaki C, Matsushima S, Watanabe T, Wada K, Nagamatsu S.
Functions of pancreatic beta cells and adipocytes in bombesin receptor subtype-3-deficient mice.
Biochem Biophys Res Commun 318(3): 698-703 [PubMed]
- Kumakura K, Sasakawa N, Murayama N, Ohara-Imaizumi M.
Spatio-temporal regulation of neurotransmitter release by PKC; studies in adrenal chromaffin cells.
Crit Rev Neurobiol 16(1-2): 173-9 [PubMed]
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2003
- Nakamichi Y, Ohara-Imaizumi M, Ishida H, Nagamatsu S.
An insulin-related peptide expressed in 3T3L1 adipocytes is localized in GLUT4 vesicles and secreted in response to exogenous insulin, which augments the insulin-stimulated glucose uptake.
J Cell Sci 116(Pt1): 73-9 [PubMed]
- Nakamichi Y, Kikuta T, Ito E, Ohara-Imaizumi M, Nishiwaki C, Ishida H, Nagamatsu S.
PPAR-gamma overexpression suppresses glucose-induced proinsulin biosynthesis and insulin release synergistically with pioglitazone in MIN6 cells.
Biochem Biophys Res Commun 306(4): 832-6 [PubMed]
- Taraska JW, Perrais D, Ohara-Imaizumi M, Nagamatsu S, Almers W.
Secretory granules are recaptured largely intact after stimulated exocytosis in cultured endocrine cells.
Proc Natl Acad Sci USA 100(4): 2070-5 [PubMed]
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2002
- Ohara-Imaizumi M, Nakamichi Y, Tanaka T, Ishida H, Nagamatsu S.
Imaging exocytosis of single insulin secretory granules with evanescent wave microscopy: distinct behavior of granule motion in biphasic insulin release.
J Biol Chem 277(6): 3805-8 [PubMed]
- Ohara-Imaizumi M, Nakamichi Y, Tanaka T, Katsuta H, Ishida H, Nagamatsu S.
Monitoring of exocytosis and endocytosis of insulin secretory granules in the pancreatic beta-cell line MIN6 using pH-sensitive green fluorescent protein (pHluorin) and confocal laser microscopy.
Biochem J 363(Pt1): 73-80 [PubMed]
- Ohara-Imaizumi M, Nakamichi Y, Nishiwaki C, Nagamatsu S.
Transduction of MIN6 beta cells with TAT-syntaxin SNARE motif inhibits insulin exocytosis in biphasic insulin release in a distinct mechanism analyzed by evanescent wave microscopy.
J Biol Chem 277(52): 50805-11 [PubMed]
- Yamada K, Wada E, Yamano M, Sun YJ, Ohara-Imaizumi M, Nagamatsu S, Wada K.
Decreased marble burying behavior in female mice lacking neuromedin-B receptor (NMB-R) implies the involvement of NMB/NMB-R in 5-HT neuron function.
Brain Res 942(1-2): 71-8 [PubMed]
- Sasakawa N, Ohara-Imaizumi M, Okubo S, Hosaka S, Hayashi M, Kumakura K.
Roles of actin filaments and the actin-myosin interaction in the regulation of exocytosis in chromaffin cells.
Ann NY Acad Sci 971: 273-4 [PubMed]
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2001
- Ohara-Imaizumi M, Nakamichi Y, Ozawa S, Katsuta H, Ishida H, Nagamatsu S.
Mastoparan stimulates GABA release from MIN6 cells: relationship between SNARE proteins and mastoparan action.
Biochem Biophys Res Commun 289(5): 1025-30 [PubMed]
- Nagamatsu S, Nakamichi Y, Ohara-Imaizumi M, Ozawa S, Katahira H, Watanabe T, Ishida H.
Adenovirus-mediated preproinsulin gene transfer into adipose tissues ameliorates hyperglycemia in obese diabetic KKA(y) mice.
FEBS Lett 509(1): 106-10 [PubMed]
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