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Faculty of Medicine教員紹介:大西 宏明

氏名 大西 宏明
オオニシ ヒロアキ
職位教授
学内の役職・委員等大学院医学研究科教務担当
研究推進センター副センター長
医学部付属病院臨床検査部長
医学部付属病院造血細胞治療センター長
所属教室

臨床検査医学教室

専攻・専門分野(大学院)

医学専攻 臨床検査医学分野(新課程)

病理系専攻 臨床検査医学分野(旧課程)

担当科目(学部)輸血、採血、がんの遺伝子検査、血液疾患の臨床検査
兼務・兼担遺伝子・染色体検査学(保健学部)
専門分野臨床検査医学、小児科学、血液腫瘍学、造血細胞治療学
研究テーマ悪性腫瘍における遺伝子異常
非結核性抗酸菌の遺伝子解析
安全な採血法の確立
略歴1990年 東京大学医学部医学科卒業
1990年 東京大学医学部小児科入局
1991年 太田西ノ内病院小児科医員
1992年 埼玉県立小児医療センター血液腫瘍科医員
1996年 東京大学小児科助手
1999年 米国ベイラー医科大学遺伝子治療研究部門研究員
2001年 杏林大学医学部臨床検査医学医学部講師
2007年 同 准教授
2015年 同 教授
現在に至る。
受賞歴:2007年臨床検査医学会学会奨励賞
所有する学位博士(医学)
指導医・専門医・認定医、その他の資格等臨床検査専門医・臨床検査管理医、日本小児科学会専門医、日本血液学会専門医・指導医、日本輸血学会認定医、日本造血・免疫細胞療法学会認定医、細胞治療認定管理師、ICD
論文・著書等を含む主要研究業績 1. Ohnishi H. Celebrating LMI Inaugural Issue. Lab Med Int 1: 3, 2022.
2. Chisada S, Ohtsuka K, Fujiwara M, Yoshida M, Matsushima S, Watanabe T, Karita K, Ohnishi H. A rad50 germline mutation induces tumorigenesis and ataxia-telangiectasia phenotype in a transparent medaka model. PLoS One 18:e0282277. doi: 10.1371/journal.pone.0282277, 2023
3. Tokunaga K, Nakamura H, Toue S, Kato Y, Ida Y, Miyoshi S, Yoneyama R, Ohnishi H, Hisamatsu T, Okamoto S. Plasma free amino acid profiles are associated with serum high molecular weight adiponectin levels in Japanese medical check‑up population without type 2 diabetes mellitus.Amino Acid. (in press)
4. Aso N, Ohtsuka K, Shibahara J, Koda H, Morikawa T, Abe N, Watanabe T, Ohnishi H. Microsatellite instability in the high-grade dysplasia component of duodenal
adenoma is associated with progression to adenocarcinoma. Surg Today 53: 252–260, 2023. doi:10.1007/s00595-022-02558-9
5. Ogura W, Ohtsuka K, Matsuura S, Okuyama T, Matsushima S, Yamasaki S, Miyagi H, Sekiguchi K, Ohnishi H, Watanabe T. Can individuals with suboptimal antibody responses to conventional antiviral vaccines acquire adequate antibodies from SARS-CoV-2 mRNA vaccination? Viruses 14:956, 2022 doi:10.3390/v14050956
6. Ida Y,Okuyama T,Araki K,Sekiguchi K,Watanabe T,Ohnishi H:First description of Lachnoanaerobaculum orale as a possible cause of human bacteremia.Anaerobe 73: 102506, 2022.
7. Yonetani S, Ohnishi H. A case of multiple abscesses caused by Nocardia farcinica rapidly diagnosed by MALDI-TOF MS. IDCases e01497. 2022.
8. Urata T, Kishino T, Watanabe K, Shibasaki S, Yotsukura M, Mori H, Kawamura N, Tanaka T, Osaka M, Matsushima S, Yamasaki S, Ohtsuka K, Ohnishi H, Watanabe T7:Sonographically measured adipose tissue thickness correlates with laboratory test abnormalities reflecting metabolic state in elderly women. Metab Syndr Relat Disord 20:148-155.2022.
9. Nakao H,Kashiwakura C,Shibasaki S,Harashima K,Nakajima S,Ohnishi H,Watanabe T,Kishino T1. Possible effects of short rest after lunch on hemodynamics in the afternoon. Eur J Appl Physiol 122: 523-530, 2022.
10. Okuyama T, Ohtsuka K, Ogura W, Yonetani S, Yamasaki S, Miyagi H, Sekiguchi K, Ohnishi H, Watanabe T. Establishing an internal quality control method for the stable extraction of nucleic acids of severe acute respiratory syndrome coronavirus 2 and RT-PCR-based detection. J Clin Lab Anal 35:e23998. doi:10.1002/jcla.23998,2021
11. Yoshida T, Kojima Y, Shimada R, Tanabe H, Tabei K, Yanagida O, Nikaido T, Ohtsuka K, Ohnishi H, Abe N, Hisamatsu T, Takahashi S. Next-generation sequencing for non-ampullary duodenal carcinoma suggesting the existence of an adenoma-carcinoma sequence. Case Rep Gastroenterol 15:62-69.doi:10.1159/000510919,2021
12. Shimamori N, Kishino T, Okabe N, Morii T, Matsushima S, Yamasaki S, Ohtsuka K, Shibahara J, Ohnishi H, Watanabe T. Discrimination of well-differentiated liposarcoma from benign lipoma on sonography: an uncontrolled retrospective study. J Med Ultrason 47:617-623,2020
13. Nozaki E, Kobayashi T, Ohnishi H, Ohtsuka K, Masaki T, Watanabe T, Sugiyama M. C-X-C motif receptor 3A enhances proliferation and invasiveness of colorectal cancer cells, and is mediated by C-X-C motif ligand 10. Oncol Lett 19:2495-2501,2020
14. Kojima Y, Ohtsuka K, Ishii S, Aso N, Ohki A, Hashimoto Y, Takeuchi H, Ohnishi H, Abe N. STK11 p.F354L Germline mutation in a case of multiple gastrointestinal tumors. Case Rep Gastroenterol 14:547-553.doi:10.1159/000508929,2020
15. Ogura W, Ohtsuka K, Fujiwara M, Tanaka R, Sekiguchi K, Ohnishi H, Watanabe T
Use of a plasma test for verifying epidermal growth factor receptor gene EGFR mutations in fluid samples from non-small cell lung cancer patients.
Respir Med Case Rep 29:101007.doi:10.1016/j.rmcr.2020.101007,2020
16. Ohtsuka K, Ohnishi H, Fujiwara M, Morii T, Matsushima S, Ogura W, Yamasaki S, Kishino T, Tanaka R, Watanabe T. Predisposition to lung adenocarcinoma in a family harboring the germline EGFR V843I mutation. JCO Precision Oncol 3:PO.19.00104.doi:10.1200/PO.19.00104,2019
17. Shimamori N, Kishino T, Morii T, Okabe N, Motohashi M, Matsushima S, Yamasaki S, Ohtsuka K, Shibahara J, Ichimura S, Ohnishi H, Watanabe T. Sonographic appearances of liposarcoma: correlations with pathologic subtypes. Ultrasound Med Biol 45:2568-2574,2019
18. Tanaka R, Sakamoto N, Suzuki H, Tachibana K, Ohtsuka K, Kishimoto K, Fujiwara M, Kamma H, Shibahara J, Kondo H. Genotyping and cytomorphological subtyping of lung adenocarcinoma based on liquid-based cytology. Diagn Cytopathol 47:564-570,2019
19. Kojima Y, Ohtsuka K, Ohnishi H, Abe N, Furuse J, Watanabe T, Sugiyama M. APC:T1556fs and STK11 mutations in duodenal adenomas and adenocarcinomas. Surg Today 48:765-772,2018
20. Aiso T, Ohtsuka K, Ueda M, Karita S, Yokoyama T, Takata S, Matsuki N, Kondo H, Takizawa H, Okada AA, Watanabe T, Ohnishi H. Serum levels of candidate microRNA diagnostic markers differ among the stages of non-small-cell lung cancer. Oncol Lett 16:6643-6651,2018
21. Yoshiyama A, Morii T, Ohtsuka K, Ohnishi H, Tajima T, Aoyagi T, Mochizuki K, Satomi K, Ichimura S. Development of stemness in cancer cell lines resistant to the anticancer effects of zoledronic acid. Anticancer Res 36:625-31,2016
22. Watanabe K, Kishino T, Sano J, Ariga T, Okuyama S, Mori H, Matsushima S, Ohtsuka K, Ohnishi H, Watanabe T. Relationship between epicardial adipose tissue thickness and early impairment of left ventricular systolic function in patients with preserved ejection fraction. Heart Vessels 31:1010-5,2016
23. Ohnishi H, Tortoli E, et al. Proposal of reclassification of Mycobacterium celatum type 2 as Mycobacterium kyorinense. Ann Microbiol 64:1879-1882, 2014
24. Ohtsuka K, Ohnishi H, et al. Whole-genome sequence of Mycobacterium kyorinense. Genome Announc 2: e01062, 2014(corresponding author)
25. Fumoto S, Ohnishi H, et al. Chimerism of buccal membrane cells in a monochorionic dizygotic twin. Pediatrics 133:e1097-1100, 2014
26. Matsushima S, Ohnishi H, et al. V843I, a lung cancer predisposing EGFR mutation, is responsible for resistance to EGFR tyrosine kinase inhibitors. J Thorac Oncol 9:1377-1384, 2014
27. Kishiki T, Ohnishi H, et al. Overexpression of MET is a new predictive marker for anti-EGFR therapy in metastatic colorectal cancer with wild-type KRAS. Cancer Chemother Pharmacol,73:749-57,2014
28. Kishiki T, Ohnishi H, et al. Impact of genetic profiles on the efficacy of anti-EGFR antibodies in metastatic colorectal cancer with KRAS mutation. Oncol Rep 32:57-64,2014
29. Morii T, Ohtsuka K, Ohnishi H, et al. BH3 mimetics inhibit growth of chondrosarcoma―a novel targeted therapy for candidate models. Anticancer Res 34:6423-30,2014
30. Kishino T, Ohnishi H, et al. Carcinosarcoma, an atypical subset of gallbladder malignancies. J Med Ultrason 41:487-490,2014
31. Ohnishi H, Yonetani S, et al. Mycobacterium kyorinense Infection: Clinical features and antimicrobial susceptibility. Emerg Infect Dis 19:508-10, 2013.
32. Aoyagi T, Ohnishi H, et al. Lung cancer cell line sensitivity to zoledronic acid is BAX-dependent. Anticancer Res 33:5357-63,2013
33. Yonetani S, Okazaki M, Ohnishi H, et al. Direct inoculation method using BacT/ALERT 3D and BD Phoenix System allows rapid and accurate identification and susceptibility testing for both Gram-positive cocci and Gram-negative rods in aerobic blood cultures. Diagn Microbiol Infect Dis 73:129-34, 2012.
34. Campos CE, Ohnishi H, Ramos JP, et al. First isolation of Mycobacterium kyorinense from clinical specimens in Brazil. J Clin Microbiol 50:2477-8,2012
35. Fukugawa Y, Ohnishi H, et al. Effect of carryover of clot activators on coagulation tests during phlebotomy. Am J Clin Pathol 137:900-3,2012
36. Ohtsuka K, Ohnishi H, et al. Familial lung adenocarcinoma caused by the EGFR V843I germ-line mutation. J Clin Oncol 29:e191-192, 2011.
37. Kishino T, Ohnishi H, et al. Low concentrations of serum n-3 polyunsaturated fatty acids in non-alcoholic fatty liver disease patients with liver injury. Clin Chem Lab Med 49:159-62,2011
38. Kishino T, Ohnishi H, et al. Subcutaneous rather than visceral adipose tissue thickness of the abdomen correlates with alterations in serum fatty acid profile in Japanese women. Clin Chim Acta 412:1893-4,2011
39. Ohtsuka K, Ohnishi H, et al. Down-regulated ABCG2 enhances sensitivity to topoisomerase I inhibitor in epidermal growth factor receptor tyrosine kinase inhibitor-resistant non-small cell lung cancer. J Thorac Oncol 5:1726-1733, 2010
40. Morii T, Ohnishi H, et al. Inhibition of heat-shock protein 27 expression eliminates drug resistance of osteosarcoma to zoledronic acid. Anticancer Res 30:3565-71,2010
41. Ohtsuka K, Ohnishi H, et al. Downregulated ABCG2 enhances sensitivity to topoisomerase I inhibitor in epidermal growth factor receptor tyrosine kinase inhibitor-resistant non-small cell lung cancer. J Thorac Oncol 5:1726-33,2010
42. Ohnishi H, Watanabe T, et al. A novel maneuver to prevent median nerve injury in phlebotomy. Ann Intern Med 151:290-1,2009.
43. Okazaki M, Ohkusu K, Ohnishi H, et al. Mycobacterium kyorinense sp. nov., a novel slowly growing Mycobacterium sp. related to Mycobacterium celatum isolated from human clinical specimens. Int J Syst Evol Microbiol 59:1336-1341; 2009.
44. Ohnishi H, Watanabe T, et al. A novel JAK2 splicing mutation in neonatal myeloproliferative disorder accompanying congenital anomalies. Brit J Haematol 145:676-678, 2009.
45. Kishino T, Ohnishi H, et al. Unusual sonographic appearance of synovial sarcoma of the anterior abdominal wall. J Clin Ultrasound,37:233-5,2009
46. Kishino T, Ohnishi H, et al. Visceral fat thickness in overweight men correlates with alterations in serum fatty acid composition. Clin Chim Acta,398:57-62,2008
47. Ohnishi H, et al. A complex t(1;22;11)(q44;q13;q23) translocation causing MLL-p300 fusion gene in therapy-related acute myeloid leukemia. Eur J Haematol 81:475-80, 2008.
48. Ohnishi H, Bessho F, et al. Faggot formation in mature neutrophils and metamyelocytes in acute myeloid leukemia without maturation. Pediatr Hematol Oncol 25:165-170,2008.
49. Onishi H, et al. Fibrin monomer complex in normal pregnant woman: a potential thrombotic marker in pregnancy. Ann Clin Biochem 44:449-454,2007.
50. Ohtsuka K, Ohnishi H, et al. Abnormalities of epidermal growth factor receptor in lung squamous-cell carcinomas, adenosquamous carcinomas, and large-cell carcinomas: tyrosine kinase domain mutations are not rare in tumors with an adenocarcinoma component. Cancer,109:741-50,2007
51. Ohnishi H, Watanabe T, et al. Chronic eosinophilic leukaemia with FIP1L1-PDFRA fusion and T674I mutation that evolved from Langerhans cell histiocytosis with eosinophilia after chemotherapy. Br J Haematol 134:544-549, 2006.
52. Ohnishi H, Watanabe T, et al. A simple and sensitive method for detecting major mutations within the tyrosine kinase domain of the epidermal growth factor receptor gene in non-small-cell lung carcinoma. Diagn Mol Pathol 15:101-108,2006.
53. Ohtsuka K, Ohnishi H, et al. Clinico-pathological and biological significance of tyrosine kinase domain gene mutations and overexpression of epidermal growth factor receptor for lung adenocarcinoma. J Thorac Oncol,1:787-95,2006
54. Ohnishi H, Bessho F, et al. Infant acute lymphoblastic leukemia with t(4;11)(q21;q23) successfully treated by immunomagnetically purged autologous bone marrow transplantation. Med Pediatr Oncol 36: 388-389, 2001
55. Yotnda P, Ohnishi H, et al. Efficient infection of primitive hematopoietic stem cells by modified adenovirus. Gene Therapy 8: 930-937, 2001
56. Ohnishi H, Taki T, et al. Down's syndrome with myelodysplastic syndrome showing t(7;11)(p13;p14). Am J Hematol 65: 62-65, 2000.
57. Guo SX, Ohnishi H, et al. Hypermethylation of p16 and p15 genes and RB protein expression in acute leukemia. Leukem Res 24: 39-46, 2000.
58. Taki T, Ohnishi H, et al. AF17q25, a putative septin family gene, fuses the MLL gene in acute myeloid leukemia with t(11;17)(q23;q25). Cancer Res 59: 4261-5, 1999
59. Bessho F, Ohnishi H, et al. Significance of electron-dense deposits in the mitochondrial matrix of erythroid precursors in aplastic anaemia and myelodysplastic syndrome. Brit Journal of Haematol 105: 149-154, 1999.
60. Kawamura M, Ohnishi H, et al. Alterations of the p53, p21, p16, p15 and RAS genes in childhood T-cell acute lymphoblastic leukemia. Leukem Res 23: 115-126, 1999.
61. Narita M, Ohnishi H, et al. Consistent detection of CALM-AF10 chimeric transcripts in haematological malignancies with t(10;11)(p13;q14) and identification of novel transcripts. Brit Journal of Haematol 105: 928-937, 1999.
62. Yang H, Ohnishi H, et al. Pattern of FHIT gene expression in normal and leukaemic cells. Int J Cancer 81: 897-901, 1999.
63. Xu F, Ohnishi H, et al. Tandem duplication of the FLT3 gene is found in acute lymphoblastic leukaemia as well as acute myeloid leukaemia but not in myelodysplastic syndrome or juvenile chronic myelogenous leukaemia in children. Brit J Haematol 105: 155-162, 1999.
64. Ohnishi H, Hayashi Y, et al. Alterations of p16 and p15 genes in acute leukemia with MLL gene rearrangements and their correlation with clinical features. Leukemia 11: 2120-24, 1997
65. Sheng X, Kawamura M, Ohnishi H, et al. Mutations of the RAS genes in childhood acute myeloid leukemia, myelodysplastic syndrome and juvenile chronic myelocytic leukemia. Leukem Res 21: 697-701, 1997.
66. Ohnishi H, Hayashi Y, et al. Infrequent mutations of the TP53 gene and no amplification of the MDM2 gene in hepatoblastomas. Genes Chromosomes Cancer 15: 187-190, 1996.
67. Ohnishi H, Hayashi Y, et al. Homozygous deletions of p16/MTS1 and p15/MTS2 genes are frequent in t(1;19)-negative- but not in t(1;19)-positive B-precursor acute lymphoblastic leukemia in childhood. Leukemia 10: 1104-1110, 1996.
68. Ohnishi H, Hayashi Y, et al. Homozygous deletions of p16/MTS1 gene are frequent but mutations are infrequent in childhood T-cell acute lymphoblastic leukemias. Blood 86: 1269-1275, 1995.


所属学会 日本臨床検査医学会(理事長)、日本輸血学会、日本小児科学会、日本検査血液学会(評議員)、日本血液学会、日本小児血液・がん学会、日本造血・免疫細胞治療学会、日本臨床微生物学会
ひとことメッセージ医学部においては、学生が良い医師になれるよう適切かつ安全な教育を行います。
医学研究科においては、大学院生の研究活動のさらなる活性化を図ります。
臨床検査部においては、検査、輸血および造血細胞治療支援が適正かつ安全に行われるよう努力してまいります。